diff --git a/src/wp-includes/block-template-utils.php b/src/wp-includes/block-template-utils.php
index 2681acb5686b3..6a4df84a11f7c 100644
--- a/src/wp-includes/block-template-utils.php
+++ b/src/wp-includes/block-template-utils.php
@@ -606,15 +606,35 @@ function _build_block_template_result_from_file( $template_file, $template_type
 		$template->area = $template_file['area'];
 	}
 
+	$hooked_blocks        = get_hooked_blocks();
+	$has_hooked_blocks    = ! empty( $hooked_blocks ) || has_filter( 'hooked_block_types' );
 	$before_block_visitor = '_inject_theme_attribute_in_template_part_block';
 	$after_block_visitor  = null;
-	$hooked_blocks        = get_hooked_blocks();
-	if ( ! empty( $hooked_blocks ) || has_filter( 'hooked_block_types' ) ) {
+
+	if ( $has_hooked_blocks ) {
 		$before_block_visitor = make_before_block_visitor( $hooked_blocks, $template, 'insert_hooked_blocks_and_set_ignored_hooked_blocks_metadata' );
 		$after_block_visitor  = make_after_block_visitor( $hooked_blocks, $template, 'insert_hooked_blocks_and_set_ignored_hooked_blocks_metadata' );
 	}
-	$blocks            = parse_blocks( $template->content );
-	$template->content = traverse_and_serialize_blocks( $blocks, $before_block_visitor, $after_block_visitor );
+
+	if ( 'wp_template_part' === $template->type && $has_hooked_blocks ) {
+		/**
+		 * In order for hooked blocks to be inserted at positions first_child and last_child in a template part,
+		 * we need to wrap its content a mock template part block and traverse it.
+		 */
+		$content           = get_comment_delimited_block_content(
+			'core/template-part',
+			array(),
+			$template->content
+		);
+		$content           = traverse_and_serialize_blocks( parse_blocks( $content ), $before_block_visitor, $after_block_visitor );
+		$template->content = remove_serialized_parent_block( $content );
+	} else {
+		$template->content = traverse_and_serialize_blocks(
+			parse_blocks( $template->content ),
+			$before_block_visitor,
+			$after_block_visitor
+		);
+	}
 
 	return $template;
 }
@@ -998,8 +1018,28 @@ function _build_block_template_result_from_post( $post ) {
 	if ( ! empty( $hooked_blocks ) || has_filter( 'hooked_block_types' ) ) {
 		$before_block_visitor = make_before_block_visitor( $hooked_blocks, $template, 'insert_hooked_blocks_and_set_ignored_hooked_blocks_metadata' );
 		$after_block_visitor  = make_after_block_visitor( $hooked_blocks, $template, 'insert_hooked_blocks_and_set_ignored_hooked_blocks_metadata' );
-		$blocks               = parse_blocks( $template->content );
-		$template->content    = traverse_and_serialize_blocks( $blocks, $before_block_visitor, $after_block_visitor );
+		if ( 'wp_template_part' === $template->type ) {
+			$existing_ignored_hooked_blocks = get_post_meta( $post->ID, '_wp_ignored_hooked_blocks', true );
+			$attributes                     = ! empty( $existing_ignored_hooked_blocks ) ? array( 'metadata' => array( 'ignoredHookedBlocks' => json_decode( $existing_ignored_hooked_blocks, true ) ) ) : array();
+
+			/**
+			 * In order for hooked blocks to be inserted at positions first_child and last_child in a template part,
+			 * we need to wrap its content a mock template part block and traverse it.
+			 */
+			$content           = get_comment_delimited_block_content(
+				'core/template-part',
+				$attributes,
+				$template->content
+			);
+			$content           = traverse_and_serialize_blocks( parse_blocks( $content ), $before_block_visitor, $after_block_visitor );
+			$template->content = remove_serialized_parent_block( $content );
+		} else {
+			$template->content = traverse_and_serialize_blocks(
+				parse_blocks( $template->content ),
+				$before_block_visitor,
+				$after_block_visitor
+			);
+		}
 	}
 
 	return $template;
@@ -1611,7 +1651,36 @@ function inject_ignored_hooked_blocks_metadata_attributes( $changes, $deprecated
 		return $template;
 	}
 
-	$changes->post_content = apply_block_hooks_to_content( $changes->post_content, $template, 'set_ignored_hooked_blocks_metadata' );
+	if ( 'wp_template_part' === $post->post_type ) {
+		$attributes                     = array();
+		$existing_ignored_hooked_blocks = isset( $post->ID ) ? get_post_meta( $post->ID, '_wp_ignored_hooked_blocks', true ) : '';
+
+		if ( ! empty( $existing_ignored_hooked_blocks ) ) {
+			$attributes['metadata'] = array(
+				'ignoredHookedBlocks' => json_decode( $existing_ignored_hooked_blocks, true ),
+			);
+		}
+
+		$content = get_comment_delimited_block_content(
+			'core/template-part',
+			$attributes,
+			$changes->post_content
+		);
+		$content = apply_block_hooks_to_content( $content, $template, 'set_ignored_hooked_blocks_metadata' );
+		$changes->post_content = remove_serialized_parent_block( $content );
+
+		$wrapper_block_markup  = extract_serialized_parent_block( $content );
+		$wrapper_block         = parse_blocks( $wrapper_block_markup )[0];
+		$ignored_hooked_blocks = $wrapper_block['attrs']['metadata']['ignoredHookedBlocks'] ?? array();
+		if ( ! empty( $ignored_hooked_blocks ) ) {
+			if ( ! isset( $changes->meta_input ) ) {
+				$changes->meta_input = array();
+			}
+			$changes->meta_input['_wp_ignored_hooked_blocks'] = wp_json_encode( $ignored_hooked_blocks );
+		}
+	} else {
+		$changes->post_content = apply_block_hooks_to_content( $changes->post_content, $template, 'set_ignored_hooked_blocks_metadata' );
+	}
 
 	return $changes;
 }
diff --git a/src/wp-includes/blocks.php b/src/wp-includes/blocks.php
index 2edcaa8c1ba04..3b1fc25d48824 100644
--- a/src/wp-includes/blocks.php
+++ b/src/wp-includes/blocks.php
@@ -1045,6 +1045,23 @@ function remove_serialized_parent_block( $serialized_block ) {
 	return substr( $serialized_block, $start, $end - $start );
 }
 
+/**
+ * Accepts the serialized markup of a block and its inner blocks, and returns serialized markup of the wrapper block.
+ *
+ * @since 6.7.0
+ * @access private
+ *
+ * @see remove_serialized_parent_block()
+ *
+ * @param string $serialized_block The serialized markup of a block and its inner blocks.
+ * @return string The serialized markup of the wrapper block.
+ */
+function extract_serialized_parent_block( $serialized_block ) {
+	$start = strpos( $serialized_block, '-->' ) + strlen( '-->' );
+	$end   = strrpos( $serialized_block, '<!--' );
+	return substr( $serialized_block, 0, $start ) . substr( $serialized_block, $end );
+}
+
 /**
  * Updates the wp_postmeta with the list of ignored hooked blocks where the inner blocks are stored as post content.
  * Currently only supports `wp_navigation` post types.
diff --git a/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromFile.php b/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromFile.php
index 3aedcf0c2c7b8..b2c2f0af75be7 100644
--- a/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromFile.php
+++ b/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromFile.php
@@ -7,6 +7,20 @@
  * @covers ::_build_block_template_result_from_file
  */
 class Tests_Block_Templates_BuildBlockTemplateResultFromFile extends WP_Block_Templates_UnitTestCase {
+	/**
+	 * Tear down each test method.
+	 *
+	 * @since 6.7.0
+	 */
+	public function tear_down() {
+		$registry = WP_Block_Type_Registry::get_instance();
+
+		if ( $registry->is_registered( 'tests/my-block' ) ) {
+			$registry->unregister( 'tests/my-block' );
+		}
+
+		parent::tear_down();
+	}
 
 	/**
 	 * @ticket 54335
@@ -178,4 +192,78 @@ public function test_should_ignore_post_types_property_when_building_template_pa
 
 		$this->assertEmpty( $template->post_types );
 	}
+
+	/**
+	 * @ticket 60506
+	 */
+	public function test_should_inject_hooked_block_into_template_part() {
+		register_block_type(
+			'tests/my-block',
+			array(
+				'block_hooks' => array(
+					'core/paragraph' => 'after',
+				),
+			)
+		);
+
+		$template_part = _build_block_template_result_from_file(
+			array(
+				'slug'      => 'header',
+				'postTypes' => array( 'post' ),
+				'path'      => DIR_TESTDATA . '/templates/template.html',
+			),
+			'wp_template_part'
+		);
+		$this->assertStringEndsWith( '<!-- wp:tests/my-block /-->', $template_part->content );
+	}
+
+	/*
+	 * @ticket 60506
+	 * @ticket 60854
+	 */
+	public function test_should_injected_hooked_block_into_template_part_first_child() {
+		register_block_type(
+			'tests/my-block',
+			array(
+				'block_hooks' => array(
+					'core/template-part' => 'first_child',
+				),
+			)
+		);
+
+		$template_part = _build_block_template_result_from_file(
+			array(
+				'slug'      => 'header',
+				'postTypes' => array( 'post' ),
+				'path'      => DIR_TESTDATA . '/templates/template.html',
+			),
+			'wp_template_part'
+		);
+		$this->assertStringStartsWith( '<!-- wp:tests/my-block /-->', $template_part->content );
+	}
+
+	/*
+	 * @ticket 60506
+	 * @ticket 60854
+	 */
+	public function test_should_injected_hooked_block_into_template_part_last_child() {
+		register_block_type(
+			'tests/my-block',
+			array(
+				'block_hooks' => array(
+					'core/template-part' => 'last_child',
+				),
+			)
+		);
+
+		$template_part = _build_block_template_result_from_file(
+			array(
+				'slug'      => 'header',
+				'postTypes' => array( 'post' ),
+				'path'      => DIR_TESTDATA . '/templates/template.html',
+			),
+			'wp_template_part'
+		);
+		$this->assertStringEndsWith( '<!-- wp:tests/my-block /-->', $template_part->content );
+	}
 }
diff --git a/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromPost.php b/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromPost.php
index 586e9beded17b..839e5788bc6c3 100644
--- a/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromPost.php
+++ b/tests/phpunit/tests/block-templates/buildBlockTemplateResultFromPost.php
@@ -111,6 +111,50 @@ public function test_should_inject_hooked_block_into_template_part() {
 		$this->assertStringEndsWith( '<!-- wp:tests/my-block /-->', $template_part->content );
 	}
 
+	/*
+	 * @ticket 59646
+	 * @ticket 60506
+	 * @ticket 60854
+	 */
+	public function test_should_injected_hooked_block_into_template_part_first_child() {
+		register_block_type(
+			'tests/my-block',
+			array(
+				'block_hooks' => array(
+					'core/template-part' => 'first_child',
+				),
+			)
+		);
+
+		$template_part = _build_block_template_result_from_post(
+			self::$template_part_post,
+			'wp_template_part'
+		);
+		$this->assertStringStartsWith( '<!-- wp:tests/my-block /-->', $template_part->content );
+	}
+
+	/*
+	 * @ticket 59646
+	 * @ticket 60506
+	 * @ticket 60854
+	 */
+	public function test_should_injected_hooked_block_into_template_part_last_child() {
+		register_block_type(
+			'tests/my-block',
+			array(
+				'block_hooks' => array(
+					'core/template-part' => 'last_child',
+				),
+			)
+		);
+
+		$template_part = _build_block_template_result_from_post(
+			self::$template_part_post,
+			'wp_template_part'
+		);
+		$this->assertStringEndsWith( '<!-- wp:tests/my-block /-->', $template_part->content );
+	}
+
 	/**
 	 * @ticket 59646
 	 * @ticket 60506
diff --git a/tests/phpunit/tests/block-templates/injectIgnoredHookedBlocksMetadataAttributes.php b/tests/phpunit/tests/block-templates/injectIgnoredHookedBlocksMetadataAttributes.php
index bc753504bbce0..1f8a66ee8546f 100644
--- a/tests/phpunit/tests/block-templates/injectIgnoredHookedBlocksMetadataAttributes.php
+++ b/tests/phpunit/tests/block-templates/injectIgnoredHookedBlocksMetadataAttributes.php
@@ -17,6 +17,7 @@ public function tear_down() {
 		if ( WP_Block_Type_Registry::get_instance()->is_registered( 'tests/hooked-block' ) ) {
 			unregister_block_type( 'tests/hooked-block' );
 		}
+		delete_post_meta( self::$template_part_post->ID, '_wp_ignored_hooked_blocks' );
 
 		parent::tear_down();
 	}
@@ -38,14 +39,35 @@ public function test_hooked_block_types_filter_with_newly_created_template() {
 
 		inject_ignored_hooked_blocks_metadata_attributes( $changes );
 
-		$args              = $action->get_args();
-		$anchor_block_type = end( $args )[2];
-		$context           = end( $args )[3];
+		$args               = $action->get_args();
+		$relative_positions = array_column( $args, 1 );
+		$anchor_block_types = array_column( $args, 2 );
+		$contexts           = array_column( $args, 3 );
 
-		$this->assertSame( 'tests/anchor-block', $anchor_block_type );
+		$this->assertSame(
+			array(
+				'before',
+				'after',
+			),
+			$relative_positions,
+			'The relative positions passed to the hooked_block_types filter are incorrect.'
+		);
 
-		$this->assertInstanceOf( 'WP_Block_Template', $context );
+		$this->assertSame(
+			array(
+				'tests/anchor-block',
+				'tests/anchor-block',
+			),
+			$anchor_block_types,
+			'The anchor block types passed to the hooked_block_types filter are incorrect.'
+		);
 
+		$context = $contexts[0];
+		$this->assertSame(
+			array_fill( 0, count( $contexts ), $context ),
+			$contexts,
+			'The context passed to the hooked_block_types filter should be the same for all calls.'
+		);
 		$this->assertSame(
 			$changes->post_type,
 			$context->type,
@@ -69,6 +91,7 @@ public function test_hooked_block_types_filter_with_newly_created_template() {
 
 	/**
 	 * @ticket 60754
+	 * @ticket 60854
 	 */
 	public function test_hooked_block_types_filter_with_newly_created_template_part() {
 		$action = new MockAction();
@@ -85,14 +108,48 @@ public function test_hooked_block_types_filter_with_newly_created_template_part(
 
 		inject_ignored_hooked_blocks_metadata_attributes( $changes );
 
-		$args              = $action->get_args();
-		$anchor_block_type = end( $args )[2];
-		$context           = end( $args )[3];
+		$args               = $action->get_args();
+		$relative_positions = array_column( $args, 1 );
+		$anchor_block_types = array_column( $args, 2 );
+		$contexts           = array_column( $args, 3 );
 
-		$this->assertSame( 'tests/anchor-block', $anchor_block_type );
+		$this->assertSame(
+			array(
+				'before',
+				'after',
+				'first_child',
+				'before',
+				'after',
+				'last_child',
+			),
+			$relative_positions,
+			'The relative positions passed to the hooked_block_types filter are incorrect.'
+		);
 
-		$this->assertInstanceOf( 'WP_Block_Template', $context );
+		$this->assertSame(
+			array(
+				'core/template-part',
+				'core/template-part',
+				'core/template-part',
+				'tests/anchor-block',
+				'tests/anchor-block',
+				'core/template-part',
+			),
+			$anchor_block_types,
+			'The anchor block types passed to the hooked_block_types filter are incorrect.'
+		);
 
+		$context = $contexts[0];
+		$this->assertSame(
+			array_fill( 0, count( $contexts ), $context ),
+			$contexts,
+			'The context passed to the hooked_block_types filter should be the same for all calls.'
+		);
+		$this->assertInstanceOf(
+			'WP_Block_Template',
+			$context,
+			'The context passed to the hooked_block_types filter is not an instance of WP_Block_Template.'
+		);
 		$this->assertSame(
 			$changes->post_type,
 			$context->type,
@@ -140,14 +197,35 @@ public function test_hooked_block_types_filter_with_existing_template_file() {
 
 		inject_ignored_hooked_blocks_metadata_attributes( $changes );
 
-		$args              = $action->get_args();
-		$anchor_block_type = end( $args )[2];
-		$context           = end( $args )[3];
+		$args               = $action->get_args();
+		$relative_positions = array_column( $args, 1 );
+		$anchor_block_types = array_column( $args, 2 );
+		$contexts           = array_column( $args, 3 );
 
-		$this->assertSame( 'tests/anchor-block', $anchor_block_type );
+		$this->assertSame(
+			array(
+				'before',
+				'after',
+			),
+			$relative_positions,
+			'The relative positions passed to the hooked_block_types filter are incorrect.'
+		);
 
-		$this->assertInstanceOf( 'WP_Block_Template', $context );
+		$this->assertSame(
+			array(
+				'tests/anchor-block',
+				'tests/anchor-block',
+			),
+			$anchor_block_types,
+			'The anchor block types passed to the hooked_block_types filter are incorrect.'
+		);
 
+		$context = $contexts[0];
+		$this->assertSame(
+			array_fill( 0, count( $contexts ), $context ),
+			$contexts,
+			'The context passed to the hooked_block_types filter should be the same for all calls.'
+		);
 		$this->assertSame(
 			$changes->post_name,
 			$context->slug,
@@ -181,6 +259,7 @@ public function test_hooked_block_types_filter_with_existing_template_file() {
 
 	/**
 	 * @ticket 60754
+	 * @ticket 60854
 	 */
 	public function test_hooked_block_types_filter_with_existing_template_part_file() {
 		$action = new MockAction();
@@ -201,14 +280,48 @@ public function test_hooked_block_types_filter_with_existing_template_part_file(
 
 		inject_ignored_hooked_blocks_metadata_attributes( $changes );
 
-		$args              = $action->get_args();
-		$anchor_block_type = end( $args )[2];
-		$context           = end( $args )[3];
+		$args               = $action->get_args();
+		$relative_positions = array_column( $args, 1 );
+		$anchor_block_types = array_column( $args, 2 );
+		$contexts           = array_column( $args, 3 );
 
-		$this->assertSame( 'tests/anchor-block', $anchor_block_type );
+		$this->assertSame(
+			array(
+				'before',
+				'after',
+				'first_child',
+				'before',
+				'after',
+				'last_child',
+			),
+			$relative_positions,
+			'The relative positions passed to the hooked_block_types filter are incorrect.'
+		);
 
-		$this->assertInstanceOf( 'WP_Block_Template', $context );
+		$this->assertSame(
+			array(
+				'core/template-part',
+				'core/template-part',
+				'core/template-part',
+				'tests/anchor-block',
+				'tests/anchor-block',
+				'core/template-part',
+			),
+			$anchor_block_types,
+			'The anchor block types passed to the hooked_block_types filter are incorrect.'
+		);
 
+		$context = $contexts[0];
+		$this->assertSame(
+			array_fill( 0, count( $contexts ), $context ),
+			$contexts,
+			'The context passed to the hooked_block_types filter should be the same for all calls.'
+		);
+		$this->assertInstanceOf(
+			'WP_Block_Template',
+			$context,
+			'The context passed to the hooked_block_types filter is not an instance of WP_Block_Template.'
+		);
 		$this->assertSame(
 			$changes->post_name,
 			$context->slug,
@@ -259,14 +372,35 @@ public function test_hooked_block_types_filter_with_existing_template_post() {
 
 		inject_ignored_hooked_blocks_metadata_attributes( $changes );
 
-		$args              = $action->get_args();
-		$anchor_block_type = end( $args )[2];
-		$context           = end( $args )[3];
+		$args               = $action->get_args();
+		$relative_positions = array_column( $args, 1 );
+		$anchor_block_types = array_column( $args, 2 );
+		$contexts           = array_column( $args, 3 );
 
-		$this->assertSame( 'tests/anchor-block', $anchor_block_type );
+		$this->assertSame(
+			array(
+				'before',
+				'after',
+			),
+			$relative_positions,
+			'The relative positions passed to the hooked_block_types filter are incorrect.'
+		);
 
-		$this->assertInstanceOf( 'WP_Block_Template', $context );
+		$this->assertSame(
+			array(
+				'tests/anchor-block',
+				'tests/anchor-block',
+			),
+			$anchor_block_types,
+			'The anchor block types passed to the hooked_block_types filter are incorrect.'
+		);
 
+		$context = $contexts[0];
+		$this->assertSame(
+			array_fill( 0, count( $contexts ), $context ),
+			$contexts,
+			'The context passed to the hooked_block_types filter should be the same for all calls.'
+		);
 		$this->assertSame(
 			$changes->post_name,
 			$context->slug,
@@ -302,6 +436,7 @@ public function test_hooked_block_types_filter_with_existing_template_post() {
 
 	/**
 	 * @ticket 60754
+	 * @ticket 60854
 	 */
 	public function test_hooked_block_types_filter_with_existing_template_part_post() {
 		$action = new MockAction();
@@ -318,14 +453,48 @@ public function test_hooked_block_types_filter_with_existing_template_part_post(
 
 		inject_ignored_hooked_blocks_metadata_attributes( $changes );
 
-		$args              = $action->get_args();
-		$anchor_block_type = end( $args )[2];
-		$context           = end( $args )[3];
+		$args               = $action->get_args();
+		$relative_positions = array_column( $args, 1 );
+		$anchor_block_types = array_column( $args, 2 );
+		$contexts           = array_column( $args, 3 );
 
-		$this->assertSame( 'tests/anchor-block', $anchor_block_type );
+		$this->assertSame(
+			array(
+				'before',
+				'after',
+				'first_child',
+				'before',
+				'after',
+				'last_child',
+			),
+			$relative_positions,
+			'The relative positions passed to the hooked_block_types filter are incorrect.'
+		);
 
-		$this->assertInstanceOf( 'WP_Block_Template', $context );
+		$this->assertSame(
+			array(
+				'core/template-part',
+				'core/template-part',
+				'core/template-part',
+				'tests/anchor-block',
+				'tests/anchor-block',
+				'core/template-part',
+			),
+			$anchor_block_types,
+			'The anchor block types passed to the hooked_block_types filter are incorrect.'
+		);
 
+		$context = $contexts[0];
+		$this->assertSame(
+			array_fill( 0, count( $contexts ), $context ),
+			$contexts,
+			'The context passed to the hooked_block_types filter should be the same for all calls.'
+		);
+		$this->assertInstanceOf(
+			'WP_Block_Template',
+			$context,
+			'The context passed to the hooked_block_types filter is not an instance of WP_Block_Template.'
+		);
 		$this->assertSame(
 			$changes->post_name,
 			$context->slug,
@@ -419,4 +588,37 @@ public function test_inject_ignored_hooked_blocks_metadata_attributes_into_templ
 			'The hooked block was not injected into the anchor block\'s ignoredHookedBlocks metadata.'
 		);
 	}
+
+	/**
+	 * @ticket 60854
+	 */
+	public function test_inject_ignored_hooked_blocks_metadata_attributes_into_template_part_postmeta() {
+		register_block_type(
+			'tests/hooked-block',
+			array(
+				'block_hooks' => array(
+					'core/template-part' => 'last_child',
+				),
+			)
+		);
+
+		$id       = self::TEST_THEME . '//' . 'my_template_part';
+		$template = get_block_template( $id, 'wp_template_part' );
+
+		$changes               = new stdClass();
+		$changes->ID           = $template->wp_id;
+		$changes->post_content = '<!-- wp:tests/anchor-block -->Hello<!-- /wp:tests/anchor-block -->';
+
+		$post = inject_ignored_hooked_blocks_metadata_attributes( $changes );
+		$this->assertSame(
+			array( 'tests/hooked-block' ),
+			json_decode( $post->meta_input['_wp_ignored_hooked_blocks'], true ),
+			'The hooked block was not injected into the wp_template_part\'s _wp_ignored_hooked_blocks postmeta.'
+		);
+		$this->assertSame(
+			$changes->post_content,
+			$post->post_content,
+			'The template part\'s post content was modified.'
+		);
+	}
 }