diff --git a/eng/testing/tests.wasi.targets b/eng/testing/tests.wasi.targets
index 7adc77cc2a7aba..1841dd20ae9a01 100644
--- a/eng/testing/tests.wasi.targets
+++ b/eng/testing/tests.wasi.targets
@@ -42,10 +42,12 @@
     <_XHarnessArgs Condition="'$(WasmXHarnessArgs)' != ''"         >$(_XHarnessArgs) $(WasmXHarnessArgs)</_XHarnessArgs>
     <_XHarnessArgs Condition="'$(_XHarnessTestsTimeout)' != ''   " >$(_XHarnessArgs) &quot;--timeout=$(_XHarnessTestsTimeout)&quot;</_XHarnessArgs>
     <_XHarnessArgs Condition="'$(WasmXHarnessArgsCli)' != ''"      >$(_XHarnessArgs) $(WasmXHarnessArgsCli)</_XHarnessArgs>
+    
+    <_InvariantGlobalization Condition="'$(InvariantGlobalization)' == 'true'">--env=DOTNET_SYSTEM_GLOBALIZATION_INVARIANT=true</_InvariantGlobalization>
 
     <!-- There two flavors of WasmXHarnessArgs and WasmXHarnessMonoArgs, one is MSBuild property and the other is environment variable -->
-    <RunScriptCommand Condition="'$(OS)' != 'Windows_NT'">$HARNESS_RUNNER $(_XHarnessArgs) %24XHARNESS_ARGS %24WasmXHarnessArgs -- $(WasmXHarnessMonoArgs) %24WasmXHarnessMonoArgs $(_AppArgs) %24WasmTestAppArgs</RunScriptCommand>
-    <RunScriptCommand Condition="'$(OS)' == 'Windows_NT'">%HARNESS_RUNNER% $(_XHarnessArgs) %XHARNESS_ARGS% %WasmXHarnessArgs%  -- $(WasmXHarnessMonoArgs) %WasmXHarnessMonoArgs% $(_AppArgs) %WasmTestAppArgs%</RunScriptCommand>
+    <RunScriptCommand Condition="'$(OS)' != 'Windows_NT'">$HARNESS_RUNNER $(_XHarnessArgs) %24XHARNESS_ARGS %24WasmXHarnessArgs -- $(WasmXHarnessMonoArgs) %24WasmXHarnessMonoArgs $(_InvariantGlobalization) %24_InvariantGlobalization  $(_AppArgs) %24WasmTestAppArgs</RunScriptCommand>
+    <RunScriptCommand Condition="'$(OS)' == 'Windows_NT'">%HARNESS_RUNNER% $(_XHarnessArgs) %XHARNESS_ARGS% %WasmXHarnessArgs%  -- $(WasmXHarnessMonoArgs) %WasmXHarnessMonoArgs% $(_InvariantGlobalization) %_InvariantGlobalization% $(_AppArgs) %WasmTestAppArgs%</RunScriptCommand>
   </PropertyGroup>
 
   <PropertyGroup Condition="'$(BuildAOTTestsOnHelix)' == 'true'">
diff --git a/src/libraries/tests.proj b/src/libraries/tests.proj
index b9896f104b24cc..47fc849ed6ede4 100644
--- a/src/libraries/tests.proj
+++ b/src/libraries/tests.proj
@@ -544,6 +544,7 @@
     <SmokeTestProject Include="$(MSBuildThisFileDirectory)System.Private.Xml.Linq\tests\Schema\System.Xml.Schema.Extensions.Tests.csproj" />
     <SmokeTestProject Include="$(MSBuildThisFileDirectory)System.Runtime.Serialization.Json\tests\System.Runtime.Serialization.Json.Tests.csproj" />
     <SmokeTestProject Include="$(MSBuildThisFileDirectory)System.Globalization\tests\System.Globalization.Tests.csproj" />
+    <SmokeTestProject Include="$(MSBuildThisFileDirectory)System.Globalization\tests\Invariant\Invariant.Tests.csproj" />
   </ItemGroup>
 
   <ItemGroup Condition="'$(TestNativeAot)' == 'true'">
diff --git a/src/mono/wasi/build/WasiApp.Native.targets b/src/mono/wasi/build/WasiApp.Native.targets
index ef10347b0341fb..c4b290bb3d327b 100644
--- a/src/mono/wasi/build/WasiApp.Native.targets
+++ b/src/mono/wasi/build/WasiApp.Native.targets
@@ -170,7 +170,6 @@
       <_WasmCommonCFlags Include="-DGEN_PINVOKE=1" />
       <_WasmCommonCFlags Condition="'$(WasmSingleFileBundle)' == 'true'" Include="-DBUNDLED_ASSEMBLIES=1" />
 
-      <_WasmCommonCFlags Include="-DINVARIANT_GLOBALIZATION=1" Condition="'$(InvariantGlobalization)' == 'true'"/>
       <!-- Adding optimization flag at the top, so it gets precedence -->
       <!--<_EmccCFlags Include="$(EmccCompileOptimizationFlag)" />-->
       <!--<_EmccCFlags Include="-s ASSERTIONS=$(_EmccAssertionLevelDefault)" Condition="'$(_WasmDevel)' == 'true'" />-->
diff --git a/src/mono/wasi/runtime/CMakeLists.txt b/src/mono/wasi/runtime/CMakeLists.txt
index cb9c20dc5308de..58d5ecaf0b15d7 100644
--- a/src/mono/wasi/runtime/CMakeLists.txt
+++ b/src/mono/wasi/runtime/CMakeLists.txt
@@ -9,11 +9,7 @@ set(CMAKE_EXECUTABLE_SUFFIX ".wasm")
 add_executable(dotnet driver.c pinvoke.c stubs.c synthetic-pthread.c)
 
 target_include_directories(dotnet PUBLIC ${MONO_INCLUDES} ${MONO_OBJ_INCLUDES} ${CMAKE_CURRENT_BINARY_DIR}/include/wasm)
-if(INVARIANT_GLOBALIZATION)
-target_compile_options(dotnet PUBLIC @${NATIVE_BIN_DIR}/src/wasi-default.rsp @${NATIVE_BIN_DIR}/src/wasi-compile.rsp -DCORE_BINDINGS -DGEN_PINVOKE=1 -DINVARIANT_GLOBALIZATION=1)
-else()
 target_compile_options(dotnet PUBLIC @${NATIVE_BIN_DIR}/src/wasi-default.rsp @${NATIVE_BIN_DIR}/src/wasi-compile.rsp -DCORE_BINDINGS -DGEN_PINVOKE=1)
-endif()
 
 set_target_properties(dotnet PROPERTIES COMPILE_FLAGS ${CONFIGURATION_WASICC_FLAGS})
 
diff --git a/src/mono/wasi/runtime/driver.c b/src/mono/wasi/runtime/driver.c
index 533b0afd4498f8..6512de66f92e05 100644
--- a/src/mono/wasi/runtime/driver.c
+++ b/src/mono/wasi/runtime/driver.c
@@ -372,11 +372,9 @@ mono_wasm_load_runtime (const char *unused, int debug_level)
 {
 	const char *interp_opts = "";
 
-#ifndef INVARIANT_GLOBALIZATION
-	load_icu_data();
-#else
-	monoeg_g_setenv ("DOTNET_SYSTEM_GLOBALIZATION_INVARIANT", "true", 1);
-#endif
+    char* invariant_globalization = monoeg_g_getenv ("DOTNET_SYSTEM_GLOBALIZATION_INVARIANT");
+    if (strcmp(invariant_globalization, "true") != 0 && strcmp(invariant_globalization, "1") != 0)
+	    load_icu_data();
 
 #ifdef DEBUG
 	monoeg_g_setenv ("MONO_LOG_LEVEL", "debug", 0);
diff --git a/src/mono/wasi/wasi.proj b/src/mono/wasi/wasi.proj
index 80f33794621467..ea89bec7d5dda7 100644
--- a/src/mono/wasi/wasi.proj
+++ b/src/mono/wasi/wasi.proj
@@ -223,7 +223,6 @@
       <CMakeBuildRuntimeConfigureCmd>$(CMakeBuildRuntimeConfigureCmd) -DICU_LIB_DIR=&quot;$(ICULibDir.TrimEnd('\/'))&quot;</CMakeBuildRuntimeConfigureCmd>
       <CMakeBuildRuntimeConfigureCmd>$(CMakeBuildRuntimeConfigureCmd) -DMONO_ARTIFACTS_DIR=&quot;$(MonoArtifactsPath.TrimEnd('\/'))&quot;</CMakeBuildRuntimeConfigureCmd>
       <CMakeBuildRuntimeConfigureCmd>$(CMakeBuildRuntimeConfigureCmd) -DNATIVE_BIN_DIR=&quot;$(NativeBinDir.TrimEnd('\/'))&quot;</CMakeBuildRuntimeConfigureCmd>
-      <CMakeBuildRuntimeConfigureCmd Condition="'$(InvariantGlobalization)' == 'true'">$(CMakeBuildRuntimeConfigureCmd) -DINVARIANT_GLOBALIZATION=1</CMakeBuildRuntimeConfigureCmd>
       <CMakeBuildRuntimeConfigureCmd Condition="'$(WasmEnableSIMD)' == 'true' and '$(Configuration)' == 'Release'">$(CMakeBuildRuntimeConfigureCmd) -DWASM_OPT_ADDITIONAL_FLAGS=&quot;--enable-simd&quot;</CMakeBuildRuntimeConfigureCmd>
       <CMakeBuildRuntimeConfigureCmd Condition="'$(MonoWasmThreads)' == 'true'">$(CMakeBuildRuntimeConfigureCmd) -DDISABLE_THREADS=0</CMakeBuildRuntimeConfigureCmd>
       <CMakeBuildRuntimeConfigureCmd Condition="'$(MonoWasmThreadsNoUser)' == 'true'">$(CMakeBuildRuntimeConfigureCmd) -DDISABLE_WASM_USER_THREADS=1</CMakeBuildRuntimeConfigureCmd>