diff --git a/README.md b/README.md
index 2cf6505be..6eb5e6930 100644
--- a/README.md
+++ b/README.md
@@ -142,7 +142,7 @@ Validator                               | Description
 **isLicensePlate(str [, locale])**     | check if string matches the format of a country's license plate.<br/><br/>(locale is one of `['cs-CZ', 'de-DE', 'de-LI', 'fi-FI', pt-PT', 'sq-AL', 'pt-BR']` or `any`)
 **isLocale(str)**                       | check if the string is a locale
 **isLowercase(str)**                    | check if the string is lowercase.
-**isMACAddress(str)**                   | check if the string is a MAC address.<br/><br/>`options` is an object which defaults to `{no_separators: false}`. If `no_separators` is true, the validator will allow MAC addresses without separators. Also, it allows the use of hyphens, spaces or dots e.g  '01 02 03 04 05 ab', '01-02-03-04-05-ab' or '0102.0304.05ab'.
+**isMACAddress(str [, options])**                   | check if the string is a MAC address.<br/><br/>`options` is an object which defaults to `{no_separators: false}`. If `no_separators` is true, the validator will allow MAC addresses without separators. Also, it allows the use of hyphens, spaces or dots e.g  '01 02 03 04 05 ab', '01-02-03-04-05-ab' or '0102.0304.05ab'. The options also allow a `eui` property to specify if it needs to be validated against EUI-48 or EUI-64. The accepted values of `eui` are: 48, 64.
 **isMagnetURI(str)**                      | check if the string is a [magnet uri format](https://en.wikipedia.org/wiki/Magnet_URI_scheme).
 **isMD5(str)**                          | check if the string is a MD5 hash.<br/><br/>Please note that you can also use the `isHash(str, 'md5')` function. Keep in mind that MD5 has some collision weaknesses compared to other algorithms (e.g., SHA).
 **isMimeType(str)**                     | check if the string matches to a valid [MIME type](https://en.wikipedia.org/wiki/Media_type) format
diff --git a/src/lib/isMACAddress.js b/src/lib/isMACAddress.js
index 8ffc8c254..d87cd4aa7 100644
--- a/src/lib/isMACAddress.js
+++ b/src/lib/isMACAddress.js
@@ -1,18 +1,34 @@
 import assertString from './util/assertString';
 
-const macAddress = /^(?:[0-9a-fA-F]{2}([-:\s]))([0-9a-fA-F]{2}\1){4}([0-9a-fA-F]{2})$/;
-const macAddressNoSeparators = /^([0-9a-fA-F]){12}$/;
-const macAddressWithDots = /^([0-9a-fA-F]{4}\.){2}([0-9a-fA-F]{4})$/;
+const macAddress48 = /^(?:[0-9a-fA-F]{2}([-:\s]))([0-9a-fA-F]{2}\1){4}([0-9a-fA-F]{2})$/;
+const macAddress48NoSeparators = /^([0-9a-fA-F]){12}$/;
+const macAddress48WithDots = /^([0-9a-fA-F]{4}\.){2}([0-9a-fA-F]{4})$/;
+const macAddress64 = /^(?:[0-9a-fA-F]{2}([-:\s]))([0-9a-fA-F]{2}\1){6}([0-9a-fA-F]{2})$/;
+const macAddress64NoSeparators = /^([0-9a-fA-F]){16}$/;
+const macAddress64WithDots = /^([0-9a-fA-F]{4}\.){3}([0-9a-fA-F]{4})$/;
 
 export default function isMACAddress(str, options) {
   assertString(str);
+  if (options?.eui) {
+    options.eui = String(options.eui);
+  }
   /**
    * @deprecated `no_colons` TODO: remove it in the next major
   */
-  if (options && (options.no_colons || options.no_separators)) {
-    return macAddressNoSeparators.test(str);
+  if (options?.no_colons || options?.no_separators) {
+    if (options.eui === '48') {
+      return macAddress48NoSeparators.test(str);
+    }
+    if (options.eui === '64') {
+      return macAddress64NoSeparators.test(str);
+    }
+    return macAddress48NoSeparators.test(str) || macAddress64NoSeparators.test(str);
   }
-
-  return macAddress.test(str)
-    || macAddressWithDots.test(str);
+  if (options?.eui === '48') {
+    return macAddress48.test(str) || macAddress48WithDots.test(str);
+  }
+  if (options?.eui === '64') {
+    return macAddress64.test(str) || macAddress64WithDots.test(str);
+  }
+  return isMACAddress(str, { eui: '48' }) || isMACAddress(str, { eui: '64' });
 }
diff --git a/test/validators.js b/test/validators.js
index a4e00293b..966351649 100644
--- a/test/validators.js
+++ b/test/validators.js
@@ -810,6 +810,14 @@ describe('Validators', () => {
         '01 02 03 04 05 ab',
         '01-02-03-04-05-ab',
         '0102.0304.05ab',
+        'ab:ab:ab:ab:ab:ab:ab:ab',
+        'FF:FF:FF:FF:FF:FF:FF:FF',
+        '01:02:03:04:05:06:07:ab',
+        '01:AB:03:04:05:06:07:08',
+        'A9 C5 D4 9F EB D3 B6 65',
+        '01 02 03 04 05 06 07 ab',
+        '01-02-03-04-05-06-07-ab',
+        '0102.0304.0506.07ab',
       ],
       invalid: [
         'abc',
@@ -822,6 +830,67 @@ describe('Validators', () => {
         '01-02 03:04 05 ab',
         '0102.03:04.05ab',
         '900f/dffs/sdea',
+        '01:02:03:04:05:06:07',
+        '01:02:03:04:05:06:07:z0',
+        '01:02:03:04:05:06::ab',
+        '1:2:3:4:5:6:7:8',
+        'AB:CD:EF:GH:01:02:03:04',
+        'A9C5 D4 9F EB D3 B6 65',
+        '01-02 03:04 05 06 07 ab',
+        '0102.03:04.0506.07ab',
+        '900f/dffs/sdea/54gh',
+      ],
+    });
+    test({
+      validator: 'isMACAddress',
+      args: [{
+        eui: '48',
+      }],
+      valid: [
+        'ab:ab:ab:ab:ab:ab',
+        'FF:FF:FF:FF:FF:FF',
+        '01:02:03:04:05:ab',
+        '01:AB:03:04:05:06',
+        'A9 C5 D4 9F EB D3',
+        '01 02 03 04 05 ab',
+        '01-02-03-04-05-ab',
+        '0102.0304.05ab',
+      ],
+      invalid: [
+        'ab:ab:ab:ab:ab:ab:ab:ab',
+        'FF:FF:FF:FF:FF:FF:FF:FF',
+        '01:02:03:04:05:06:07:ab',
+        '01:AB:03:04:05:06:07:08',
+        'A9 C5 D4 9F EB D3 B6 65',
+        '01 02 03 04 05 06 07 ab',
+        '01-02-03-04-05-06-07-ab',
+        '0102.0304.0506.07ab',
+      ],
+    });
+    test({
+      validator: 'isMACAddress',
+      args: [{
+        eui: '64',
+      }],
+      valid: [
+        'ab:ab:ab:ab:ab:ab:ab:ab',
+        'FF:FF:FF:FF:FF:FF:FF:FF',
+        '01:02:03:04:05:06:07:ab',
+        '01:AB:03:04:05:06:07:08',
+        'A9 C5 D4 9F EB D3 B6 65',
+        '01 02 03 04 05 06 07 ab',
+        '01-02-03-04-05-06-07-ab',
+        '0102.0304.0506.07ab',
+      ],
+      invalid: [
+        'ab:ab:ab:ab:ab:ab',
+        'FF:FF:FF:FF:FF:FF',
+        '01:02:03:04:05:ab',
+        '01:AB:03:04:05:06',
+        'A9 C5 D4 9F EB D3',
+        '01 02 03 04 05 ab',
+        '01-02-03-04-05-ab',
+        '0102.0304.05ab',
       ],
     });
   });
@@ -837,6 +906,10 @@ describe('Validators', () => {
         'FFFFFFFFFFFF',
         '0102030405ab',
         '01AB03040506',
+        'abababababababab',
+        'FFFFFFFFFFFFFFFF',
+        '01020304050607ab',
+        '01AB030405060708',
       ],
       invalid: [
         'abc',
@@ -852,6 +925,56 @@ describe('Validators', () => {
         '01020304ab',
         '123456',
         'ABCDEFGH0102',
+        '01:02:03:04:05:06:07',
+        '01:02:03:04:05:06::ab',
+        '1:2:3:4:5:6:7:8',
+        'AB:CD:EF:GH:01:02:03:04',
+        'ab:ab:ab:ab:ab:ab:ab:ab',
+        'FF:FF:FF:FF:FF:FF:FF:FF',
+        '01:02:03:04:05:06:07:ab',
+        '01:AB:03:04:05:06:07:08',
+        '01020304050607',
+        '010203040506ab',
+        '12345678',
+        'ABCDEFGH01020304',
+      ],
+    });
+    test({
+      validator: 'isMACAddress',
+      args: [{
+        no_separators: true,
+        eui: '48',
+      }],
+      valid: [
+        'abababababab',
+        'FFFFFFFFFFFF',
+        '0102030405ab',
+        '01AB03040506',
+      ],
+      invalid: [
+        'abababababababab',
+        'FFFFFFFFFFFFFFFF',
+        '01020304050607ab',
+        '01AB030405060708',
+      ],
+    });
+    test({
+      validator: 'isMACAddress',
+      args: [{
+        no_separators: true,
+        eui: '64',
+      }],
+      valid: [
+        'abababababababab',
+        'FFFFFFFFFFFFFFFF',
+        '01020304050607ab',
+        '01AB030405060708',
+      ],
+      invalid: [
+        'abababababab',
+        'FFFFFFFFFFFF',
+        '0102030405ab',
+        '01AB03040506',
       ],
     });
   });